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Oxford Instruments
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Healthscan Products Inc
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Herley Industries
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Eiken Chemical
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ANSYS inc
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Axion BioSystems
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MetaMorph Inc
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Interacoustics AS
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TeraRecon
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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain
doi: 10.3389/fncel.2026.1756677
Figure Lengend Snippet: Intravenous antibody labeling distinguishes CNS-resident from circulating immune cells in ACSA2 + fractions. (A) Mice received I.V. injection of fluorescent antibody 3 min before tissue harvest and brain dissociation. I.V.-labeled cells represent circulating immune cells, while unlabeled CD45 + cells are CNS-resident. Created in BioRender. Daniele, E. (2026) https://BioRender.com/20anz0d . (B) Flow cytometry using anti-CD45.2-PE (I.V.) shows 20.3% of ACSA2 + cells are CD45-BV605 + /CD45.2-PE – , indicating CNS-resident immune cell contamination, while only 1.54% are circulating immune cells (CD45-BV605 + /CD45.2-PE + ). (C) Isotype control using IgG2b-κ-PE (I.V.) shows minimal non-specific labeling confirming specificity of CD45.2 labeling. Gates set on live, nucleated DRAQ5+/DAPI- singlets. Representative plots from n = 3 mice per condition.
Article Snippet: Intensity thresholds were set based on background signal measured in secondary-only and a
Techniques: Antibody Labeling, Injection, Labeling, Flow Cytometry, Control